Komara, Liris Lis and Murtinah, Veronika Mikroorganism population in reclamation and pre-mining area at PT Kaltim Prima Coal, East Kalimantan, Indonesia. In: International Conference on Biodiversity.
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Abstrak Mikroorganism population in reclamation.pdf Download (2MB) |
Abstract
Coal mining activities change environmental conditions physically, chemically and biologically. Repairing efforts to damage caused by mining must be carried out. To return the soil microorganisms, pre-mining data meeded as reference. This study aims to determine the species of bacteria and fungi in the pre-mining and unrevegetated reclamation area at Kaltim Prima Coal Company. This research was conducted in April 2021. At each location, 20 point/ha of soil samples were taken and mixed evenly, than 100 g was taken and stored in a cooler boxto keep it below 4°C. Bacteria and fungi were isolated by the spread plate method by serial dilution technique. The cultures were incubated at 28°C for 24-28 hours. The isolated fungi were then identified based on colony morphology and microscopic characteristics. The 24 hour old bacteria were stained and observed under a microscope. Then 16 biochemical tests were carried out, the data obtained were then referred to the Bergeys manual of determinative bacteriology to the genus level. The results of the study obtained 14 species of bacteria and 7 species of fungi at pre-mining and 3 species of bacteria and 2 species of fungi were obtained unrevegetated. The same bacteria that held at both location is Bacillus sp.
| Item Type: | Conference or Workshop Item (Speech) |
|---|---|
| Subjects: | S Agriculture > SD Forestry |
| Depositing User: | Perpustakaan Pusat STIPER KUTAI TIMUR |
| Date Deposited: | 20 Jul 2023 03:40 |
| Last Modified: | 20 Jul 2023 03:40 |
| URI: | https://repositori.stiperkutim.ac.id/id/eprint/218 |
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